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Azospirillum Endophyticum sp. nov., an Endophyte of Paris Polyphylla Smith var. Yunnanensis.
- Source :
-
Current microbiology [Curr Microbiol] 2022 Dec 12; Vol. 80 (1), pp. 34. Date of Electronic Publication: 2022 Dec 12. - Publication Year :
- 2022
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Abstract
- A Gram-negative, facultative anaerobic bacterial strain, designated YIM B02556 <superscript>T</superscript> , was isolated from the root of Paris polyphylla Smith var. yunnanensis collected from Yunnan Province, southwest China. By using a polyphasic approach, its taxonomic position was investigated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM B02556 <superscript>T</superscript> belonged to the genus Azospirillum and the 16S rRNA gene sequence similarity values of strain YIM B02556 <superscript>T</superscript> to the type strains of members of this genus ranged from 94.9 to 98.3%. Overall genome relatedness index (OGRI) analysis estimated based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between YIM B02556 <superscript>T</superscript> and other Azospirillum species type strains were <90.8% and <37.8%, lower than the limit of species circumscription. Cells of the strain were characterized as oxidase- and catalase-positive, with motility provided by flagella. The growth conditions of the strain were found to occur at 20-40 °C (optimum, 35 °C), and pH 6.0-9.5 (optimum, pH 7.5). Strain YIM B02556 <superscript>T</superscript> can tolerate 2% NaCl concentration. Strain YIM B02556 <superscript>T</superscript> contained Q-10 as the major ubiquinone. The major fatty acids were C <subscript>18:1</subscript> ω7c and summed feature three (C <subscript>16:1</subscript> ω7c and/or C <subscript>16:1</subscript> ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on polyphasic analysis, strain YIM B02556 <superscript>T</superscript> could be differentiated genotypically and phenotypically from recognized species of the genus Azospirillum. Therefore, the isolate represents a novel species, for which the name Azospirillum endophyticum is proposed. The type strain is YIM B02556 <superscript>T</superscript> (=JCM 34631 <superscript>T</superscript> =CGMCC 1.18654 <superscript>T</superscript> ).<br /> (© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Details
- Language :
- English
- ISSN :
- 1432-0991
- Volume :
- 80
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Current microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 36508041
- Full Text :
- https://doi.org/10.1007/s00284-022-03142-7