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Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia ( Oreochromis niloticus ) against Streptococcus agalatiae Infection.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Nov 28; Vol. 23 (23). Date of Electronic Publication: 2022 Nov 28. - Publication Year :
- 2022
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Abstract
- Vasoactive intestinal peptide (VIP), a member of secretin/glucagon family, is involved in a variety of biological activities such as gut motility, immune responses, and carcinogenesis. In this study, the VIP precursor gene ( On-VIP ) and its receptor gene VIPR1 ( On-VIPR1 ) were identified from Nile tilapia ( Oreochromis niloticus ), and the functions of On-VIP in the immunomodulation of Nile tilapia against bacterial infection were investigated and characterized. On-VIP and On-VIPR1 contain a 450 bp and a 1326 bp open reading frame encoding deduced protein of 149 and 441 amino acids, respectively. Simultaneously, the transcript of both On-VIP and On-VIPR1 were highly expressed in the intestine and sharply induced by Streptococcus agalatiae . Moreover, the positive signals of On-VIP and On-VIPR1 were detected in the longitudinal muscle layer and mucosal epithelium of intestine, respectively. Furthermore, both in vitro and in vivo experiments indicated several immune functions of On-VIP, including reduction of P65 , P38 , MyD88 , STAT3 , and AP1 , upregulation of CREB and CBP , and suppression of inflammation. Additionally, in vivo experiments proved that On-VIP could protect Nile tilapia from bacterial infection and promote apoptosis and pyroptosis. These data lay a theoretical basis for further understanding of the mechanism of VIP guarding bony fish against bacterial infection.
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 36499231
- Full Text :
- https://doi.org/10.3390/ijms232314895