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Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid.
- Source :
-
Acta cirurgica brasileira [Acta Cir Bras] 2022 Nov 28; Vol. 37 (9), pp. e370902. Date of Electronic Publication: 2022 Nov 28 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds.<br />Methods: The ingredients of WO were detected by gas chromatography-mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium.<br />Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05).<br />Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation.
- Subjects :
- Animals
Swine
Ointments
Linoleic Acid pharmacology
Wound Healing
Accidents
Juglans
Subjects
Details
- Language :
- English
- ISSN :
- 1678-2674
- Volume :
- 37
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Acta cirurgica brasileira
- Publication Type :
- Academic Journal
- Accession number :
- 36449813
- Full Text :
- https://doi.org/10.1590/acb370902