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Anti-tyrosinase and antioxidant activity of meroterpene bakuchiol from Psoralea corylifolia (L.).
- Source :
-
Food chemistry [Food Chem] 2023 Mar 30; Vol. 405 (Pt B), pp. 134953. Date of Electronic Publication: 2022 Nov 17. - Publication Year :
- 2023
-
Abstract
- Bakuchiol is gaining major interest for treatments against skin photoaging. The kinetics of mushroom tyrosinase inhibition by bakuchiol, by real-time oxygen sensing and UV-vis monitoring (475 nm), showed competitive inhibition with average K <subscript>i</subscript> constant (µM, 30 °C, pH 6.8) of 6.71 ± 1.23 and 1.15 ± 0.34 for monophenolase and diphenolase reactions respectively, with respective IC <subscript>50</subscript> 37.22 ± 5.18 and 6.91 ± 0.96 ∼ at 1 mM substrate, compared to kojic acid IC <subscript>50</subscript> 34.02 ± 5.51 and 16.86 ± 3.28 μM. Fluorescence quenching showed a single binding mode with formation constant K <subscript>a</subscript> 1.02 × 10 <superscript>6</superscript> M <superscript>-1</superscript> . The antioxidant activity was studied by inhibited autoxidation of styrene and cumene (PhCl, 30 °C) affording inhibition constant k <subscript>inh</subscript>  = 18.1 ± 6.6 (10 <superscript>4</superscript> M <superscript>-1</superscript> s <superscript>-1</superscript> , 30 °C) and of MeLin in Triton™ X-100 micelles giving k <subscript>inh</subscript>  = 0.16 ± 0.03 (10 <superscript>4</superscript> M <superscript>-1</superscript> s <superscript>-1</superscript> , 37 °C). Stoichiometric factor was 1.9 ± 0.1. ReqEPR spectroscopy afforded the BDE(OH) as 81.7 ± 0.1 kcal/mol. Bakuchiol is a potent tyrosinase inhibitor with good antioxidant activity having major potential as natural food preservative against oxidation and food-browning.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Subjects :
- Monophenol Monooxygenase
Antioxidants pharmacology
Phenols
Psoralea
Fabaceae
Subjects
Details
- Language :
- English
- ISSN :
- 1873-7072
- Volume :
- 405
- Issue :
- Pt B
- Database :
- MEDLINE
- Journal :
- Food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36423556
- Full Text :
- https://doi.org/10.1016/j.foodchem.2022.134953