Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines.

Authors :: De Ricco R
Rech F
Onnis V
Coccone SS
Scalia G
Marcozzi C
Gavini M
Beni S
Giannini S
Nompari L
Parlati C
Magagnoli C
Cianetti S
Berti F
Source :: ACS omega [ACS Omega] 2022 Oct 27; Vol. 7 (44), pp. 39875-39883. Date of Electronic Publication: 2022 Oct 27 (Print Publication: 2022).
Publication Year :: 2022
Abstract: GlaxoSmithKline (GSK) is currently developing a fully liquid presentation to ease the administration of the licensed quadrivalent conjugate vaccine (Menveo) against meningococcal serogroup A, C, W, and Y (MenACWY) infections. Herein, we report a new method for determining the free saccharide (FS) content of CRM <subscript>197</subscript> -MenACWY conjugated antigens, with the aim of improving accuracy and reproducibility. Mathematical models have been used to support technical knowledge in reducing the need for experimental development. This results in an improved, faster, and platform-based technique for FS separation with one single pretreatment applicable to all antigens of the multivalent meningococcal vaccine.<br />Competing Interests: The authors declare the following competing financial interest(s): All the authors are employees of the GSK group of companies. Francesco Berti is listed as an inventor on patents owned by the GSK group of companies.<br /> (© 2022 The Authors. Published by American Chemical Society.)

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