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Performance evaluation of UF-4000 body fluid mode for detection of bacteria in body fluids.
- Source :
-
Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2023 Jan 01; Vol. 538, pp. 9-14. Date of Electronic Publication: 2022 Oct 29. - Publication Year :
- 2023
-
Abstract
- Background: Microbiological analysis of body fluids (BF) provides important information for diagnosis of infection. We evaluated the analytical performance of bacterial count by UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ambulatory peritoneal dialysis fluids compared to classical microbiological procedure (direct Gram staining and culture).<br />Materials and Methods: For the 1,734 BF analyzed, distribution of UF-4000 bacterial count was analyzed according to the level of growth culture and results were compared using Mann-Whitney test. ROC curves analysis allowed to define the best cut-off value to predict or exclude positive culture for each type of BF.<br />Results: UF-4000 bacterial counts were significantly lower in sterile than in infected BFs (p < 0.00001) and correlated with the level of growth on culture. The ROC curves of bacteria/μL and culture positivity yielded area under the curve >0.80 for each type of BF. Optimal cut-offs were chosen with excellent statistical parameters (sensitivity ranging from 0.70 to 0.86, specificity from 0.78 to 0.98, negative predictive value >0.95 and Youden index >0.55).<br />Conclusion: For BF, UF-4000 bacterial count correlate with culture results and is a discriminative method enhancing detection of microbiological etiology. It could be used as a screening method based on the cut-off values proposed in this study.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022. Published by Elsevier B.V.)
Details
- Language :
- English
- ISSN :
- 1873-3492
- Volume :
- 538
- Database :
- MEDLINE
- Journal :
- Clinica chimica acta; international journal of clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 36374847
- Full Text :
- https://doi.org/10.1016/j.cca.2022.10.020