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In Vitro Analysis of Thymocyte Signaling.

Authors :
Teixeiro E
Daniels MA
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2580, pp. 303-313.
Publication Year :
2023

Abstract

When a developing thymocyte expresses a TCR, it is subjected to numerous interactions with self-peptide/MHC complexes that determine its fate. These include death by neglect, negative selection (apoptosis and lineage deviation), positive selection, and lineage commitment. Identifying signals that govern these unique cell fates requires the ability to assess the activity, level of expression, subcellular location, and molecular associations between numerous proteins within the developing T cell. Given the unique, temporal, and developmental changes that occur during development, isolating and analyzing small populations of thymocytes are necessary to get a complete picture of the development process. Thus, this chapter describes methods designed to analyze thymocyte signaling under various types of peptide-based stimulation in vitro.<br /> (© 2023. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2580
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
36374466
Full Text :
https://doi.org/10.1007/978-1-0716-2740-2_19