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[CircRNA-0028171 regulates arsenic trioxide-induced apoptosis in vascular endothelial cells].
- Source :
-
Sheng li xue bao : [Acta physiologica Sinica] [Sheng Li Xue Bao] 2022 Oct 25; Vol. 74 (5), pp. 763-772. - Publication Year :
- 2022
-
Abstract
- The present study was aimed to investigate the effects of circRNA-0028171 on the apoptosis of vascular endothelial cells induced by arsenic trioxide (As <subscript>2</subscript> O <subscript>3</subscript> ). Human umbilical vein endothelial cells (HUVECs) were treated with 0-15 μmol/L As <subscript>2</subscript> O <subscript>3</subscript> for 24 h. Then, cellular viability was measured by MTT assay. The expression levels of circRNA-0028171, Bcl-2 and Bax mRNA were detected by real-time quantitative PCR. Bcl-2/Bax protein ratio was detected by Western blot. Whether circRNA-0028171 was involved in the regulation of HUVECs by As <subscript>2</subscript> O <subscript>3</subscript> was investigated by transfection with overexpression plasmid of circRNA-0028171 and siRNA. The results showed that compared with the control group, As <subscript>2</subscript> O <subscript>3</subscript> group showed decreased cellular viability, reduced Bcl-2/Bax mRNA and protein ratios, and significantly lower expression of circRNA-0028171. Overexpression of circRNA-0028171 inhibited apoptosis of HUVECs induced by As <subscript>2</subscript> O <subscript>3</subscript> . Knockdown of circRNA-0028171 by siRNA promoted As <subscript>2</subscript> O <subscript>3</subscript> -induced apoptosis in HUVECs. These results suggest that circRNA-0028171 is involved in the vascular endothelial cell apoptosis induced by As <subscript>2</subscript> O <subscript>3</subscript> .
Details
- Language :
- Chinese
- ISSN :
- 0371-0874
- Volume :
- 74
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Sheng li xue bao : [Acta physiologica Sinica]
- Publication Type :
- Academic Journal
- Accession number :
- 36319099