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Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads.
- Source :
-
MicroPublication biology [MicroPubl Biol] 2022 Oct 07; Vol. 2022. Date of Electronic Publication: 2022 Oct 07 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1).<br /> (Copyright: © 2022 by the authors.)
Details
- Language :
- English
- ISSN :
- 2578-9430
- Volume :
- 2022
- Database :
- MEDLINE
- Journal :
- MicroPublication biology
- Publication Type :
- Academic Journal
- Accession number :
- 36281316
- Full Text :
- https://doi.org/10.17912/micropub.biology.000648