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General control nonderepressible 1 interacts with cationic amino acid transporter 1 and affects Aedes aegypti fecundity.
- Source :
-
Parasites & vectors [Parasit Vectors] 2022 Oct 21; Vol. 15 (1), pp. 383. Date of Electronic Publication: 2022 Oct 21. - Publication Year :
- 2022
-
Abstract
- Background: The amino acid transporter protein cationic amino acid transporter 1 (CAT1) is part of the nutrient sensor in the fat body of mosquitoes. A member of the SLC7 family of cationic amino acid transporters, it is paramount for the detection of elevated amino acid levels in the mosquito hemolymph after a blood meal and the subsequent changes in gene expression in the fat body.<br />Methods: We performed a re-annotation of Aedes aegypti cationic amino acid transporters (CATs) and selected the C-terminal tail of CAT1 to perform a yeast two-hybrid screen to identify putative interactors of this protein. One interesting interacting protein we identified was general control nonderepressible 1 (GCN1). We determined the expression pattern of GCN1 in several adult organs and structures using qRT-PCR and western blots. Finally, we knocked down GCN1 using double-stranded RNA and identified changes in downstream signaling intermediates and the effects of knockdown on vitellogenesis and fecundity.<br />Results: In a screen for Ae. aegypti CAT1-interacting proteins we identified GCN1 as a putative interactor. GCN1 is highly expressed in the ovaries and fat body of the mosquito. We provide evidence that eukaryotic translation initiation factor 2 subunit alpha (eIF2α) phosphorylation changed during vitellogenesis and that RNA interference knockdown of GCN1 in whole mosquitoes reduced egg clutch sizes of treated mosquitoes relative to controls.<br />Conclusions: Aedes aegypti CAT1 and GCN1 are likely interacting partners and GCN1 is likely necessary for proper egg development. Our data suggest that GCN1 is part of a nutrient sensor mechanism in various mosquito tissues involved in vitellogenesis.<br /> (© 2022. The Author(s).)
- Subjects :
- Animals
Cationic Amino Acid Transporter 1 genetics
Cationic Amino Acid Transporter 1 metabolism
RNA, Double-Stranded metabolism
Prokaryotic Initiation Factor-2 genetics
Prokaryotic Initiation Factor-2 metabolism
Saccharomyces cerevisiae genetics
Amino Acid Transport Systems, Basic genetics
Amino Acid Transport Systems, Basic metabolism
Amino Acids genetics
Fertility
Aedes genetics
Aedes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1756-3305
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasites & vectors
- Publication Type :
- Academic Journal
- Accession number :
- 36271393
- Full Text :
- https://doi.org/10.1186/s13071-022-05461-x