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Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide.
- Source :
-
FEBS letters [FEBS Lett] 2022 Dec; Vol. 596 (23), pp. 3024-3036. Date of Electronic Publication: 2022 Oct 31. - Publication Year :
- 2022
-
Abstract
- Glycosylinositol phosphoceramide (GIPC) is a major sphingolipid in the plasma membranes of plants. Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of the D position of GIPC in cabbage and named this activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as a PLD. The recombinant NPC3 protein obtained by heterologous expression system in Escherichia coli produced PC1P from GIPC and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish and Arabidopsis thaliana. From these results, we conclude that NPC3 is one of the enzymes that degrade GIPC.<br /> (© 2022 Federation of European Biochemical Societies.)
Details
- Language :
- English
- ISSN :
- 1873-3468
- Volume :
- 596
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 36266963
- Full Text :
- https://doi.org/10.1002/1873-3468.14520