The promising role of hypoxia-resistant insulin-producing cells in ameliorating diabetes mellitus in vivo .

Authors :: Ahmed HH
Aglan HA
Beherei HH
Mabrouk M
Mahmoud NS
Source :: Future science OA [Future Sci OA] 2022 Sep 14; Vol. 8 (7), pp. FSO811. Date of Electronic Publication: 2022 Sep 14 (Print Publication: 2022).
Publication Year :: 2022
Abstract: Aim: This study aimed to evaluate the efficacy of hypoxia-persistent insulin-producing cells (IPCs) against diabetes in vivo .<br />Materials & Methods: Mesenchymal stem cells (MSCs) differentiation into IPCs in the presence of Se/Ti (III) or CeO <subscript>2</subscript> nanomaterials. IPCs were subjected to hypoxia and hypoxia genes were analyzed. PKH-26-labeled IPCs were infused in diabetic rats to evaluate their anti-diabetic potential.<br />Results: MSCs were differentiated into functional IPCs. IPCs exhibited overexpression of anti-apoptotic genes and down-expression of hypoxia and apoptotic genes. IPCs implantation elicited glucose depletion and elevated insulin, HK and G6PD levels. They provoked VEGF and PDX-1 upregulation and HIF-1α and Caspase-3 down-regulation. IPCs transplantation ameliorated the destabilization of pancreatic tissue architecture.<br />Conclusion: The chosen nanomaterials were impressive in generating hypoxia-resistant IPCs that could be an inspirational strategy for curing diabetes.<br /> (© 2022 The Authors.)

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