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Effects of ethanamizuril, sulfachlorpyridazine or their combination on cecum microbial community and metabolomics in chickens infected with Eimeria tenella.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2022 Dec; Vol. 173 (Pt A), pp. 105823. Date of Electronic Publication: 2022 Oct 12. - Publication Year :
- 2022
-
Abstract
- Coccidiostat and antibiotics are widely used in poultry industry, but their effects on cecum microbial community and metabolomics in chickens infected with coccidia have been rarely studied. In this study, we analyzed the changes of microbiota and metabolomic which associated with Eimeria tenella infection in 8 days of age chickens in the presence or absence of ethanamizuril, sulfachlorpyridazine or their combinations treatment for 3 consecutive days. 16S rRNA gene sequencing and LC-MS/MS analyses were used to profile the cecal microbiome and metabolome in each group of chickens on 7 days post-infection. The results showed that coccidial infection induced significant perturbations in the distribution of microbial taxonomy and the metabolism of physiological functional molecules in cecal contents. Ethanamizuril treatment seemed to transform microbiota into a steady state conducive to animal health, and sulfachlorpyridazine treatment alleviated the growth of potentially harmful bacteria such as Escherichia-Shigella. The change trends of metabolites such as n-carbamoylglutamic acid were consistent with the anticoccidial effect of ethanamizuril. The combinations of ethanamizuril and sulfachlorpyridazine at low-dose had little effect on gut microbiota, metabolism and anticoccidial effect. These data indicate that the cecal microbiota and metabolic status of chickens infected with E. tenella following ethanamizuril treatment could be used to monitor the response to drug efficacy. This study provides a new system approach to elucidate the microbiota, metabolic and therapeutic effects of the combination of coccidiostat and antibiotics in the context of avian coccidiosis.<br />Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Chickens microbiology
RNA, Ribosomal, 16S genetics
Chromatography, Liquid
Tandem Mass Spectrometry
Cecum microbiology
Metabolomics
Anti-Bacterial Agents pharmacology
Anti-Bacterial Agents therapeutic use
Eimeria tenella genetics
Sulfachlorpyridazine therapeutic use
Coccidiostats pharmacology
Coccidiostats therapeutic use
Poultry Diseases drug therapy
Poultry Diseases microbiology
Coccidiosis drug therapy
Coccidiosis veterinary
Gastrointestinal Microbiome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 173
- Issue :
- Pt A
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 36243384
- Full Text :
- https://doi.org/10.1016/j.micpath.2022.105823