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Histochemical assessment of accelerated bone remodeling and reduced mineralization in Il-6 deficient mice.
- Source :
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Journal of oral biosciences [J Oral Biosci] 2022 Dec; Vol. 64 (4), pp. 410-421. Date of Electronic Publication: 2022 Oct 12. - Publication Year :
- 2022
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Abstract
- Objectives: Interleukin-6 (IL-6) contributes to the regulation of functions in various tissues and organs. Even though IL-6 has been reported to modulate bone metabolism in previous studies, this finding is controversial. This study aims to evaluate the possible involvement of IL-6 in bone metabolism by examining the histological activity of osteoblasts and osteoclasts in the femora of Il-6 deficient (Il-6 <superscript>-/-</superscript> ) mice.<br />Methods: Eight-week-old male Il-6 <superscript>-/-</superscript> mice and their wild-type littermates were fixed with a paraformaldehyde solution, and their femora were extracted for micro-CT analysis, immunohistochemistry, and real-time PCR analysis.<br />Results: Il-6 <superscript>-/-</superscript> femora showed an increased bone volume/tissue volume (TV) but a reduced bone mineral density compared with the wild-type. Furthermore, the tissue-nonspecific alkaline phosphatase positive area/TV ratio, the expression of Runx2, Osterix, and Rankl, and the number of tartrate-resistant acid phosphatase-positive osteoclasts were all increased in the Il-6 <superscript>-/-</superscript> mice. A considerable number of unmineralized areas within the bone matrix and abundant sclerostin-reactive osteocytes were observed in Il-6 <superscript>-/-</superscript> femoral metaphyses but not in the wild-type. Interestingly, the gene expression of Cd206 was elevated in Il-6 <superscript>-/-</superscript> femora, and many F4/80-positive macrophages/monocytes and CD206-immunoreactive macrophages in the primary trabeculae had migrated closer to the growth plate, where intense RANKL immunoreactivity was detected. These results suggest that, in an IL-6-deficient state, CD206-positive macrophages may differentiate into osteoclasts when in contact with RANKL-reactive osteoblastic cells.<br />Conclusion: In a state of IL-6 deficiency, the population and cell activities of osteoblast, osteoclasts, and macrophages seemed to be facilitated, except for the reduced mineralization in bone.<br />Competing Interests: Conflicts of interest No potential conflicts of interest exist.<br /> (Copyright © 2022 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1880-3865
- Volume :
- 64
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of oral biosciences
- Publication Type :
- Academic Journal
- Accession number :
- 36241157
- Full Text :
- https://doi.org/10.1016/j.job.2022.10.001