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Germline immortality relies on TRIM32-mediated turnover of a maternal mRNA activator in C. elegans .
- Source :
-
Science advances [Sci Adv] 2022 Oct 14; Vol. 8 (41), pp. eabn0897. Date of Electronic Publication: 2022 Oct 14. - Publication Year :
- 2022
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Abstract
- How the germ line achieves a clean transition from maternal to zygotic gene expression control is a fundamental problem in sexually reproducing organisms. Whereas several mechanisms terminate the maternal program in the soma, this combined molecular reset and handover are poorly understood for primordial germ cells (PGCs). Here, we show that GRIF-1, a TRIM32-related and presumed E3 ubiquitin ligase in Caenorhabditis elegans , eliminates the maternal cytoplasmic poly(A) polymerase (cytoPAP) complex by targeting the germline-specific intrinsically disordered region of its enzymatic subunit, GLD-2, for proteasome-mediated degradation. Interference with cytoPAP turnover in PGCs causes frequent transgenerational sterility and, eventually, germline mortality. Hence, positively acting maternal RNA regulators are cleared via the proteasome system to avoid likely interference between maternal and zygotic gene expression programs to maintain transgenerational fertility and acquire germline immortality. This strategy is likely used in all animals that preform their immortal germ line via maternally inherited germplasm determinants.
- Subjects :
- Animals
Germ Cells metabolism
Proteasome Endopeptidase Complex metabolism
RNA metabolism
RNA, Messenger, Stored genetics
RNA, Messenger, Stored metabolism
Ubiquitin-Protein Ligases genetics
Ubiquitin-Protein Ligases metabolism
Caenorhabditis elegans genetics
Caenorhabditis elegans metabolism
Caenorhabditis elegans Proteins genetics
Caenorhabditis elegans Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2375-2548
- Volume :
- 8
- Issue :
- 41
- Database :
- MEDLINE
- Journal :
- Science advances
- Publication Type :
- Academic Journal
- Accession number :
- 36240265
- Full Text :
- https://doi.org/10.1126/sciadv.abn0897