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WNT1 Inducible Signaling Pathway Protein 1 Is a Stroma-Specific Secreting Protein Inducing a Fibroblast Contraction and Carcinoma Cell Growth in the Human Prostate.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Sep 28; Vol. 23 (19). Date of Electronic Publication: 2022 Sep 28. - Publication Year :
- 2022
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Abstract
- The WNT1 inducible signaling pathway protein 1 (WISP1), a member of the connective tissue growth factor family, plays a crucial role in several important cellular functions in a highly tissue-specific manner. Results of a RT-qPCR indicated that WISP1 expressed only in cells of the human prostate fibroblasts, HPrF and WPMY-1, but not the prostate carcinoma cells in vitro. Two major isoforms (WISP1v1 and WISP1v2) were identified in the HPrF cells determined by RT-PCR and immunoblot assays. The knock-down of a WISP1 blocked cell proliferation and contraction, while treating respectively with the conditioned medium from the ectopic WISP1v1- and WISPv2-overexpressed 293T cells enhanced the migration of HPrF cells. The TNFα induced WISP1 secretion and cell contraction while the knock-down of WISP1 attenuated these effects, although TNFα did not affect the proliferation of the HPrF cells. The ectopic overexpression of WISP1v1 but not WISP1v2 downregulated the N-myc downstream regulated 1 (NDRG1) while upregulating N-cadherin, slug, snail, and vimentin gene expressions which induced not only the cell proliferation and invasion in vitro but also tumor growth of prostate carcinoma cells in vivo. The results confirmed that WISP1 is a stroma-specific secreting protein, enhancing the cell migration and contraction of prostate fibroblasts, as well as the proliferation, invasion, and tumor growth of prostate carcinoma cells.
- Subjects :
- Cadherins
Carcinoma metabolism
Carcinoma pathology
Cell Proliferation genetics
Cell Proliferation physiology
Connective Tissue Growth Factor
Culture Media, Conditioned pharmacology
Humans
Male
Prostate metabolism
Prostate pathology
Signal Transduction
Tumor Necrosis Factor-alpha pharmacology
Vimentin metabolism
CCN Intercellular Signaling Proteins genetics
CCN Intercellular Signaling Proteins metabolism
Cell Transformation, Neoplastic genetics
Cell Transformation, Neoplastic metabolism
Fibroblasts metabolism
Fibroblasts pathology
Prostatic Neoplasms genetics
Prostatic Neoplasms pathology
Proto-Oncogene Proteins genetics
Proto-Oncogene Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 36232736
- Full Text :
- https://doi.org/10.3390/ijms231911437