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Fluorescence Correlation Spectroscopy and Phase Separation.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2563, pp. 161-198. - Publication Year :
- 2023
-
Abstract
- A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Spectrum Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2563
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 36227473
- Full Text :
- https://doi.org/10.1007/978-1-0716-2663-4_8