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Editing of StSR4 by Cas9-RNPs confers resistance to Phytophthora infestans in potato.
- Source :
-
Frontiers in plant science [Front Plant Sci] 2022 Sep 23; Vol. 13, pp. 997888. Date of Electronic Publication: 2022 Sep 23 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Potato ( Solanum tuberosum L.) cultivation is threatened by various environmental stresses, especially disease. Genome editing technologies are effective tools for generating pathogen-resistant potatoes. Here, we established an efficient RNP-mediated CRISPR/Cas9 genome editing protocol in potato to develop Phytophthora infestans resistant mutants by targeting the susceptibility gene, Signal Responsive 4 ( SR4 ), in protoplasts. Mutations in StSR4 were efficiently introduced into the regenerated potato plants, with a maximum efficiency of 34%. High co-expression of StEDS1 and StPAD4 in stsr4 mutants induced the accumulation of salicylic acid (SA), and enhanced the expression of the pathogen resistance marker StPR1 . In addition, increased SA content in the stsr4 mutant enhanced its resistance to P. infestans more than that in wild type. However, the growth of stsr4&#95;3-19 and stsr4&#95;3-698 mutants with significantly high SA was strongly inhibited, and a dwarf phenotype was induced. Therefore, it is important to adequate SA accumulation in order to overcome StSR4 editing-triggered growth inhibition and take full advantages of the improved pathogen resistance of stsr4 mutants. This RNP-mediated CRISPR/Cas9-based potato genome editing protocol will accelerate the development of pathogen-resistant Solanaceae crops via molecular breeding.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Moon, Park, Park, Lee, Shin, Lee, Choi, Kim, Cho, Jeon, Kim, Park and Kim.)
Details
- Language :
- English
- ISSN :
- 1664-462X
- Volume :
- 13
- Database :
- MEDLINE
- Journal :
- Frontiers in plant science
- Publication Type :
- Academic Journal
- Accession number :
- 36212382
- Full Text :
- https://doi.org/10.3389/fpls.2022.997888