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Interaction of MyoD and MyoG with Myoz2 gene in bovine myoblast differentiation.

Authors :
Wei D
Zhang J
Raza SHA
Song Y
Jiang C
Song X
Wu H
Alotaibi MA
Albiheyri R
Al-Zahrani M
Makhlof RTM
Alsaad MA
Abdelnour SA
Quan G
Source :
Research in veterinary science [Res Vet Sci] 2022 Dec 20; Vol. 152, pp. 569-578. Date of Electronic Publication: 2022 Sep 26.
Publication Year :
2022

Abstract

This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (-159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region -159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.<br />Competing Interests: Declaration of Competing Interest The authors have declared that no competing interest exists.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1532-2661
Volume :
152
Database :
MEDLINE
Journal :
Research in veterinary science
Publication Type :
Academic Journal
Accession number :
36191510
Full Text :
https://doi.org/10.1016/j.rvsc.2022.09.023