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Hawthorn fruit extract ameliorates H 2 O 2 -induced oxidative damage in neuronal PC12 cells and prolongs the lifespan of Caenorhabditis elegans via the IIS signaling pathway.
- Source :
-
Food & function [Food Funct] 2022 Oct 17; Vol. 13 (20), pp. 10680-10694. Date of Electronic Publication: 2022 Oct 17. - Publication Year :
- 2022
-
Abstract
- Hawthorn ( Crataegus pinnatifida ) fruit has a long history of use as traditional Chinese medicine and is shown to have many health benefits including antioxidant and anti-aging. In this study, the anti-aging mechanism of hawthorn fruit extract (HFE) is predicted by network pharmacology and further verified in H <subscript>2</subscript> O <subscript>2</subscript> -induced PC12 cells and Caenorhabditis elegans . Network pharmacology predicted that the antiaging mechanism of HFE is mainly involved in phosphoinositide 3-kinase (PI3K)/AKT and the insulin/insulin-like growth factor-1 (IIS) signaling pathway. HFE significantly improved cell viability, increased superoxide dismutase, catalase, and glutathione peroxidase activity, decreased lactate dehydrogenase release, the level of reactive oxygen species (ROS), and malondialdehyde content in H <subscript>2</subscript> O <subscript>2</subscript> -induced PC12 cells ( p < 0.05). HFE significantly increased the mean lifespan of C. elegans by 28.43% (100 μg mL <superscript>-1</superscript> ) and enhanced the stress resistance to H <subscript>2</subscript> O <subscript>2</subscript> , paraquat, juglone, ultraviolet radiation, and heat shock. HFE also suppressed the accumulation of aging pigments, improved the body bending ability, increased antioxidant enzyme activities, and reduced the contents of ROS and malondialdehyde. In addition, relevant gene expression, lifespan experiments with mutant strains, and molecular docking studies supported the results that HFE might extend lifespan through the IIS signal pathway.
- Subjects :
- Animals
Antioxidants metabolism
Antioxidants pharmacology
Caenorhabditis elegans genetics
Catalase metabolism
Fruit metabolism
Glutathione Peroxidase metabolism
Hydrogen Peroxide metabolism
Hydrogen Peroxide toxicity
Insulin-Like Growth Factor I metabolism
Lactate Dehydrogenases metabolism
Longevity
Malondialdehyde metabolism
Molecular Docking Simulation
Oxidative Stress
PC12 Cells
Paraquat
Phosphatidylinositol 3-Kinase metabolism
Phosphatidylinositol 3-Kinases genetics
Phosphatidylinositol 3-Kinases metabolism
Plant Extracts metabolism
Plant Extracts pharmacology
Proto-Oncogene Proteins c-akt metabolism
Rats
Reactive Oxygen Species metabolism
Signal Transduction
Superoxide Dismutase metabolism
Ultraviolet Rays
Crataegus
Insulins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2042-650X
- Volume :
- 13
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Food & function
- Publication Type :
- Academic Journal
- Accession number :
- 36172739
- Full Text :
- https://doi.org/10.1039/d2fo01657e