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Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088.

Authors :
Cheli F
Falsini S
Salvatici MC
Ristori S
Schiff S
Corti E
Costantini I
Gonnelli C
Pavone FS
Papini A
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2566, pp. 345-353.
Publication Year :
2023

Abstract

The microscopic visualization of nanoparticles in plants is crucial to elucidate the mechanisms of their uptake through the cell wall and plasma membrane and to localize the possible sites of their extracellular or intracellular accumulation. Lignin nanocarriers are polymeric hollow nanocapsules able to contain and transport several bioactive substances inside plant tissues. We describe here a method for the preparation of Fluorol Yellow 088-labeled lignin nanocapsules that allow their localization in plant organs and tissues by fluorescence microscopy.<br /> (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2566
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
36152265
Full Text :
https://doi.org/10.1007/978-1-0716-2675-7_28