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Hsa_circ_0003602 Contributes to the Progression of Colorectal Cancer by Mediating the miR-149-5p/SLC38A1 Axis.
- Source :
-
Gut and liver [Gut Liver] 2023 Mar 15; Vol. 17 (2), pp. 267-279. Date of Electronic Publication: 2022 Sep 23. - Publication Year :
- 2023
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Abstract
- Background/aims: We aimed to investigate the role and working mechanism of Homo sapiens circular RNA&#95;0003602 (hsa&#95;circ&#95;0003602) in colorectal cancer (CRC) development.<br />Methods: The expression of circ&#95;0003602, miR-149-5p, and solute carrier family 38 member 1 (SLC38A1) was detected by quantitative real-time polymerase chain reaction. RNase R assays were conducted to determine the characteristics of circ&#95;0003602. CCK-8 assays, flow cytometry analysis, transwell invasion assays, wound healing assays and tube formation assays were employed to evaluate cell viability, apoptosis, invasion, migration, and angiogenesis. All protein levels were examined by Western blot or immunohistochemistry assay. The glutamine metabolism was monitored by corresponding glutamine, α-ketoglutarate and glutamate assay kits. Dual-luciferase reporter assay was utilized to confirm the targeted combination between miR-149-5p and circ&#95;0003602 or SLC38A1. A xenograft tumor model was established to analyze the role of circ&#95;0003602 in CRC tumor growth in vivo.<br />Results: Circ&#95;0003602 was upregulated in CRC tissues and cell lines. Circ&#95;0003602 silencing suppressed CRC cell viability, migration, invasion, angiogenesis, and glutaminolysis; induced cell apoptosis in vitro; and blocked tumor growth in vivo. Moreover, circ&#95;0003602 directly interacted with miR-149-5p to negatively regulate its expression, and circ&#95;0003602 knockdown suppressed the malignant behaviors of CRC cells largely by upregulating miR-149-5p. MiR-149-5p directly bound to the 3' untranslated region of SLC38A1 to induce its degradation, and miR-149-5p overexpression reduced the malignant potential of CRC cells largely by downregulating SLC38A1. Circ&#95;0003602 positively regulated SLC38A1 expression by sponging miR-149-5p in CRC cells.<br />Conclusions: Circ&#95;0003602 knockdown impedes CRC development by targeting the miR-149-5p/SLC38A1 axis, which provides a novel theoretical basis and new insights for CRC treatment.
Details
- Language :
- English
- ISSN :
- 2005-1212
- Volume :
- 17
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gut and liver
- Publication Type :
- Academic Journal
- Accession number :
- 36148577
- Full Text :
- https://doi.org/10.5009/gnl210542