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Pig Coat Color Manipulation by MC1R Gene Editing.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2022 Sep 08; Vol. 23 (18). Date of Electronic Publication: 2022 Sep 08. - Publication Year :
- 2022
-
Abstract
- Black coat color in pigs is determined by the dominant E allele at the MC1R locus. Through comparing MC1R gene sequences between recessive e and dominant E <superscript>D1</superscript> alleles, we identified four missense mutations that could affect MC1R protein function for eumelanin synthesis. With the aim of devising a genetic modification method for pig coat color manipulation, we mutated the e allele in the Duroc breed to the dominant E <superscript>D1</superscript> allele using CRISPR-mediated homologous recombination for the four mutation substitutions at the MC1R locus. The MC1R -modified Duroc pigs generated using the allele replacement strategy displayed uniform black coat color across the body. A genotyping assay showed that the MC1R -modified Duroc pigs had a heterozygous E <superscript>D1</superscript> / e allele at the MC1R locus; in addition, the pigs remained in the Duroc genetic background. Our work offers a gene editing method for pig coat color manipulation, which could value the culture of new pig varieties meeting the needs of diversified market.
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 23
- Issue :
- 18
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 36142269
- Full Text :
- https://doi.org/10.3390/ijms231810356