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Arabinoxylan as well as β-glucan in barley promotes GLP-1 secretion by increasing short-chain fatty acids production.

Authors :
Mio K
Ogawa R
Tadenuma N
Aoe S
Source :
Biochemistry and biophysics reports [Biochem Biophys Rep] 2022 Sep 13; Vol. 32, pp. 101343. Date of Electronic Publication: 2022 Sep 13 (Print Publication: 2022).
Publication Year :
2022

Abstract

Barley is rich in soluble dietary fiber including β-glucan and arabinoxylan. Barley β-glucan is fermented by gut bacteria and, thereby contributes to an effect on intestinal bacterial composition and short-chain fatty acids (SCFAs). It also increases GLP-1 secretion via SCFAs receptor. However, few studies have focused on barley arabinoxylan. Therefore, we have investigated the effects of arabinoxylan from barley on intestinal fermentability and GLP-1 secretion. C57BL/6J mice were fed a high-fat diet containing arabinoxylan-dominant barley flour without β-glucan (bgl) and high β-glucan-containing barley flour (BF) for 12 weeks. We conducted oral glucose tolerance test (OGTT) to measure insulin and GLP-1 concentrations. The concentration of SCFAs in the cecum contents was also determined. Furthermore, we measured mRNA expression assay GLP-1 secretion using real-time PCR. The OGTT result showed that GLP-1 concentrations at 60 min were increased in mice fed bgl and BF. Acetic acid and total SCFAs concentrations in the cecum contents were increased in both the barley groups, and butyric acid was increased in the bgl group. Furthermore, the bgl and BF groups had increased Gpr43, a receptor for SCFAs, and NeuroD which is involved in L cell differentiation. These results show arabinoxylan as well as β-glucan is involved in the SCFAs-mediated increase in GLP-1 secretion upon barley consumption.<br />Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Seiichiro Aoe reports financial support was provided by Hakubaku Co., Ltd. Kento Mio reports a relationship with Hakubaku Co., Ltd that includes: employment.<br /> (© 2022 The Authors.)

Details

Language :
English
ISSN :
2405-5808
Volume :
32
Database :
MEDLINE
Journal :
Biochemistry and biophysics reports
Publication Type :
Academic Journal
Accession number :
36123993
Full Text :
https://doi.org/10.1016/j.bbrep.2022.101343