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Protocol for the isolation of CD8+ tumor-infiltrating lymphocytes from human tumors and their characterization by single-cell immune profiling and multiome.
- Source :
-
STAR protocols [STAR Protoc] 2022 Aug 26; Vol. 3 (3), pp. 101649. Date of Electronic Publication: 2022 Aug 26 (Print Publication: 2022). - Publication Year :
- 2022
-
Abstract
- Understanding the heterogenicity of tumor-infiltraing lymphocyte (TIL) populations and the immunobiology in human cancer is a key to establish efficient immunotherapies. Here, we have established a protocol for the characterization of CD8 <superscript>+</superscript> TILs in tumors by single-cell RNA-seq paired to VDJ profiling and chromatin structure including dissociation of tumor biopsies. We have also provided guidance for subsequent fluorescence-activated cell sorting (FACS), single-cell encapsulation, bioinformatics analysis, and troubleshooting. For complete details on the use and execution of this protocol, please refer to Anadon et al. (2022).<br />Competing Interests: J.R.C.-G. has stock options in Compass Therapeutics, Anixa Biosciences, and Alloy Therapeutics; has sponsored research with Anixa Biosciences; receives honorarium from Alloy Therapeutics and Leidos; and has intellectual property with Compass Therapeutics and Anixa Biosciences.<br /> (© 2022 The Author(s).)
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 3
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 36065294
- Full Text :
- https://doi.org/10.1016/j.xpro.2022.101649