Back to Search
Start Over
Role of salts and solvents on the defibrillation of food dye "sunset yellow" induced hen egg white lysozyme amyloid fibrils.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2022 Oct 31; Vol. 219, pp. 1351-1359. Date of Electronic Publication: 2022 Sep 02. - Publication Year :
- 2022
-
Abstract
- Several food dyes are known to induce amyloid fibrillation when interacting with proteins. Here, we studied the role of sunset yellow (SY) in the amyloid fibrillation of hen egg white lysozyme (HEWL) and characterized the changes using spectroscopy techniques. Turbidity results showed that SY dye induces aggregation in HEWL in concentrations dependent manner. The aggregation induced by SY dye is kinetically very fast, no lag phase was detected, and the kinetics process follows an isodesmic kinetics pathway. The SY-dye induce aggregates have cross-β secondary structure confirmed by far-UV CD measurements. The effect of salts and solvents was also seen on SY-induced aggregates. Turbidity, far-UV CD, and kinetics results suggest that certain concentrations of NaCl and (NH <subscript>4</subscript> ) <subscript>2</subscript> SO <subscript>4</subscript> solubilize the SY-induce amyloid fibrils, but (NH <subscript>4</subscript> ) <subscript>2</subscript> SO <subscript>4</subscript> is more effective. Similarly, solvents are also solubilized the SY-induces HEWL amyloid fibrillation but the order of defibrillation is as follows: Isopropanol> ethanol > methanol which signified that isopropanol is more effective than other solvents. The salts and solvents data suggest that the electrostatic, as well as hydrophobic interaction, is responsible for SY-induced amyloid fibrillation. These conformational changes should be examined, more seriously for the purpose of food safety.<br />Competing Interests: Declaration of competing interest None.<br /> (Copyright © 2022 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 219
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 36058397
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2022.08.199