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Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression.
- Source :
-
Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Aug 11; Vol. 10, pp. 878311. Date of Electronic Publication: 2022 Aug 11 (Print Publication: 2022). - Publication Year :
- 2022
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Abstract
- Duchenne Muscular Dystrophy (DMD) is an X-linked neuromuscular disease which to date is incurable. The major cause of death is dilated cardiomyopathy however, its pathogenesis is unclear as existing cellular and animal models do not fully recapitulate the human disease phenotypes. In this study, we generated cardiac organoids from patient-derived induced pluripotent stem cells (DMD-COs) and isogenic-corrected controls (DMD-Iso-COs) and studied if DMD-related cardiomyopathy and disease progression occur in the organoids upon long-term culture (up to 93 days). Histological analysis showed that DMD-COs lack initial proliferative capacity, displayed a progressive loss of sarcoglycan localization and high stress in endoplasmic reticulum. Additionally, cardiomyocyte deterioration, fibrosis and aberrant adipogenesis were observed in DMD-COs over time. RNA sequencing analysis confirmed a distinct transcriptomic profile in DMD-COs which was associated with functional enrichment in hypertrophy/dilated cardiomyopathy, arrhythmia, adipogenesis and fibrosis pathways. Moreover, five miRNAs were identified to be crucial in this dysregulated gene network. In conclusion, we generated patient-derived cardiac organoid model that displayed DMD-related cardiomyopathy and disease progression phenotypes in long-term culture. We envision the feasibility to develop a more complex, realistic and reliable in vitro 3D human cardiac-mimics to study DMD-related cardiomyopathies.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Marini, Marino, Aliberti, Giarratana, Pozzo, Duelen, Cortés Calabuig, La Rovere, Vervliet, Torella, Bultynck, Sampaolesi and Chai.)
Details
- Language :
- English
- ISSN :
- 2296-634X
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- Frontiers in cell and developmental biology
- Publication Type :
- Academic Journal
- Accession number :
- 36035984
- Full Text :
- https://doi.org/10.3389/fcell.2022.878311