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Conserved Role of mTORC1 Signaling in B Cell Immunity in Teleost Fish.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2022 Sep 15; Vol. 209 (6), pp. 1095-1107. Date of Electronic Publication: 2022 Aug 19. - Publication Year :
- 2022
-
Abstract
- Mammalian studies have demonstrated that B cell immune responses are regulated by mechanistic target of rapamycin complex 1 (mTORC1) signaling. Teleost fish represent the oldest living bony vertebrates that contain bona fide B cells. So far, whether the regulatory mechanism of mTORC1 signaling in B cells occurred in teleost fish is still unknown. In this study, we developed a fish model by using rapamycin (RAPA) treatment to inhibit mTORC1 signaling and demonstrated the role of mTORC1 signaling in teleost B cells. In support, we found inhibition of mTORC1 signaling by RAPA decreased the phagocytic capacity, proliferation, and Ig production of B cells. Critically, Flavobacterium columnare induced specific IgM binding in serum, and these titers were significantly inhibited by RAPA treatment, thus decreasing Ab-mediated agglutination of F. columnare and significantly increasing the susceptibility of fish upon F. columnare reinfection. Collectively, our findings elucidated that the mTORC1 pathway is evolutionarily conserved in regulating B cell responses, thus providing a new point for understanding the B cells functions in teleost fish.<br /> (Copyright © 2022 by The American Association of Immunologists, Inc.)
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 209
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 35985789
- Full Text :
- https://doi.org/10.4049/jimmunol.2200280