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Pathogenic effects of Desulfovibrio in the gut on fatty liver in diet-induced obese mice and children with obesity.
- Source :
-
Journal of gastroenterology [J Gastroenterol] 2022 Nov; Vol. 57 (11), pp. 913-925. Date of Electronic Publication: 2022 Aug 17. - Publication Year :
- 2022
-
Abstract
- Background: Although we know the key role of gut dysbiosis in nonalcoholic fatty liver disease (NAFLD), it remains unclear what microbe(s) are responsible. This study aims to identify the microbes that cause NAFLD.<br />Methods: C57BL/6JNarl male mice fed a high-fat diet (HFD) were orally administered Lactobacillus reuteri (L. reuteri) or Lactobacillus rhamnosus GG plus Bifidobacterium animalis subsp. lactis BB12 (LGG plus BB12). Their fecal microbiomes identified by 16S rRNA sequencing were correlated with the severity of fatty liver. We then used a human cohort to confirm the role of the microbe(s). The HFD-fed mice were administrated with the identified bacterium, Desulfovibrio. The histopathological changes in the liver and ileum were analyzed.<br />Results: Lactobacillus and Bifidobacterium improved hepatic steatosis and fibrosis in HFD-fed mice, which was related to the decreased abundance of Desulfovibrio in feces. Further human study confirmed the amount of D. piger in the fecal microbiota of obese children with NAFLD was increased. We then administered D. piger and found aggravated hepatic steatosis and fibrosis in HFD-fed mice. Hepatic expression of CD36 was significantly increased in HFD-fed mice gavaged with D. piger. In HepG2 cells, overexpression of CD36 increased lipid droplets, whereas knockdown of CD36 decreased lipid droplets. HFD-fed mice gavaged with D. piger had a decrease in the villus length, crypt depth, and zonula occludens-1 density in the ileum tissue.<br />Conclusions: Our findings provide novel insights into the role of Desulfovibrio dysregulation in NAFLD. Modulation of Desulfovibrio may be a potential target for the treatment of NAFLD.<br /> (© 2022. Japanese Society of Gastroenterology.)
- Subjects :
- Child
Male
Humans
Mice
Animals
Mice, Obese
RNA, Ribosomal, 16S
Mice, Inbred C57BL
Diet, High-Fat adverse effects
Liver pathology
Liver Cirrhosis pathology
Non-alcoholic Fatty Liver Disease pathology
Gastrointestinal Microbiome genetics
Pediatric Obesity complications
Pediatric Obesity metabolism
Pediatric Obesity pathology
Desulfovibrio genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1435-5922
- Volume :
- 57
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 35976494
- Full Text :
- https://doi.org/10.1007/s00535-022-01909-0