[Comparative evaluation of the diagnostic value of indirect and reverse ELISA for demonstrations of specific IgM antibodies in toxoplasmosis in man].

Two ELISA techniques were investigated comparatively regarding their IgM sensitivity and specificity in human toxoplasmosis. Results of the fractionated Toxo-IgM-IIF assay were used as a standard for comparison. In 96 of 264 specimens the Toxo-IgM-IIF assay was positive. Positive sera were investigated quantitatively, negative sera only in the serum dilution 1/50 in both the indirect and the reverse Toxo-IgM ELISA. Sensitivity of the indirect technique was calculated greater than 99% and specificity 97%. The corresponding values for the reverse technique were 66% for sensitivity and greater than 99% for specificity.