Back to Search
Start Over
Within- and cross-tissue gene regulations were disrupted by PM 2.5 nitrate exposure and associated with respiratory functions.
- Source :
-
The Science of the total environment [Sci Total Environ] 2022 Dec 01; Vol. 850, pp. 157977. Date of Electronic Publication: 2022 Aug 11. - Publication Year :
- 2022
-
Abstract
- Background: Pathogenesis of complex diseases often involves multiple organs/tissue-types. To date, the PM <subscript>2.5</subscript> exposure's toxic effects and induced disease risks were not studied at multi-tissue level.<br />Methods: C57BL/6 mice (n = 40) were exposed to PM <subscript>2.5</subscript> NO <subscript>3</subscript> <superscript>-</superscript> and clean air, respectively, and afterwards assessed respiratory functions and transcriptome in relevant tissues: blood and lung. We constructed within- and cross-tissue gene regulation networks and identified network modules associated with exposure and respiratory functions.<br />Results: PM <subscript>2.5</subscript> NO <subscript>3</subscript> <superscript>-</superscript> exposure elevated naïve B cells proportion in blood (p = 0.0028). Among the 6000 highest expressed genes in blood, 18.8 % (1133 genes) were altered by exposure at p ≤ 0.05 level, among which 763 genes were also associated with respiratory function (enrichment folds = 7.63, p = 2.7E-189). The exposure disrupted blood genes were primarily in the immunoregulation pathways. Both within- and cross-tissue gene network modules were perturbed by exposure and associated with respiratory function. An immunodeficiency related cross-tissue module of 555 genes was affected by exposure (p = 0.0023) and strongly correlated with FEV <subscript>0.05</subscript> /FVC (r = 0.61 and p = 3E-5).<br />Conclusions: This study aims to fill in a major knowledge gap and investigated the effect of PM <subscript>2.5</subscript> exposure simultaneously in multiple tissues. We provided novel evidence that PM <subscript>2.5</subscript> NO <subscript>3</subscript> <superscript>-</superscript> exposure profoundly perturbed within- and cross-tissue gene regulations, and highlighted their roles in the etiology of respiratory decline.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-1026
- Volume :
- 850
- Database :
- MEDLINE
- Journal :
- The Science of the total environment
- Publication Type :
- Academic Journal
- Accession number :
- 35964746
- Full Text :
- https://doi.org/10.1016/j.scitotenv.2022.157977