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Promoting effect of Fe 3+ on gentamicin resistance in Escherichia coli.

Authors :: Huang YF
Li Y
Chen JY
Lin JH
Liu L
Ye JZ
Su YB
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Oct 15; Vol. 625, pp. 134-139. Date of Electronic Publication: 2022 Aug 04.
Publication Year :: 2022
Abstract: Kinds of antibiotics are used to prevent and control bacteria infections, unfortunately, the overuse and misuse of antibiotic have promoted the emergence and spread of antibiotic-resistant bacteria. Therefore, understanding the mechanism of antibiotic resistance is very important. This study explores the combined effection of metal ions and antibiotic to the drug resistance of Escherichia coli. Our results found that the minimum inhibitory concentration (MIC) increased as the ammonium ferric citrate concentration increased, especially for gentamicin antibiotic. When the Fe <superscript>3+</superscript> concentration reached 300 μM, the survival of E. coli was stably restored with the increased gentamicin concentration. Exogenous Fe <superscript>3+</superscript> could decrease intracellular gentamicin concentration. On the other hand, Fe <superscript>3+</superscript> treatment together with gentamicin could reduce reactive oxygen species (ROS) production, characterized by decreased levels of NADH and ATP. Furthermore, ROS-scavenging enzymes of superoxide dismutase (SOD) and catalase (CAT) were up-regulated and H <subscript>2</subscript> O <subscript>2</subscript> plus gentamicin-mediated killing was restored by Fe <superscript>3+</superscript> . These results may have significant implications in understanding bacterial antibiotic-resistant mechanisms based on the external Fe <superscript>3+</superscript> concentration.<br />Competing Interests: Declaration of competing interest The authors declare there is no conflict of interest.<br /> (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)