Start Over

Anti-inflammatory withajardins from the leaves of Athenaea velutina.

Authors :: Dantas Rocha KA
de Freitas Paulo T
Ayala AP
da Silva Sampaio V
Gomes Nunes PI
Santos FA
Canuto KM
Silveira ER
Loiola Pessoa OD
Source :: Phytochemistry [Phytochemistry] 2022 Nov; Vol. 203, pp. 113338. Date of Electronic Publication: 2022 Aug 07.
Publication Year :: 2022
Abstract: Withajardins, uncommon modified withanolide-type steroids, have been isolated exclusively from plants of the Solanaceae family so far. Two undescribed withajardins and the known tuboanosigenin were isolated from the hexane/EtOAc 1:1 extract from Athenaea velutina leaves. Their structures were established by an extensive analysis of 1D and 2D-NMR and HRMS data. The absolute configuration was determined by X-ray diffraction (withajardin L and tuboanosigenin) and circular dichroism (CD) analyses (withajardin M). The anti-inflammatory activity of compounds was evaluated through the inhibition of the lipopolysaccharide (LPS)-induced nitric oxide (NO), TNF-α, and IL-6 release in RAW264.7 cells. The cell viability effects to RAW 264.7 cells showed IC <subscript>50</subscript> values of 74.4-354.4 μM. The compounds attenuated LPS-induced release of NO and decreased pro-inflammatory cytokines TNF-α and IL-6 in RAW264.7 cells.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)