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Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid-producing Alternaria alternata on livestock feeds.
- Source :
-
Journal of applied microbiology [J Appl Microbiol] 2022 Nov; Vol. 133 (5), pp. 3020-3029. Date of Electronic Publication: 2022 Aug 10. - Publication Year :
- 2022
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Abstract
- Aims: This study aims to demonstrate the potential of the lactic acid bacteria (LAB) Pediococcus pentosaceus LBM18 against the mycotoxin-producing Alternaria alternata TEF-1A and highlight its application as an effective grain silage inoculant to control mycotoxin contamination.<br />Methods and Results: The antifungal properties of Ped. pentosaceus lyophilized (PPL) were assessed by evaluating its effect on A. alternata TEF-1A grown in a corn silage-based medium, which included morphological changes by Scanning Electron Microscopy (SEM) observations, growth rate, conidia production assays, and inhibition of Tenuazonic acid (TeA) production by high-performance liquid chromatography (HPLC-MS/MS) analyses. Furthermore, TeA biosynthesis was monitored for changes at the molecular level by PKS gene expression. The growth and sporulation processes of A. alternata TEF-1A were affected by Ped. pentosaceus LBM18 in a concentration-dependent manner. Moreover, a significant inhibition of TeA production (74.3%) and the transcription level of the PKS gene (42.9%) was observed.<br />Conclusions: Ped. pentosaceus is one of the promising LAB to be applied as an inoculant for corn silage preservation, aiming to inhibit mycotoxigenic fungi growth and their mycotoxin production.<br />Significance and Impact of the Study: Ped. pentosaceus could be used as an inoculant to reduce fungal and mycotoxins contamination in grain silage production.<br /> (© 2022 Society for Applied Microbiology.)
Details
- Language :
- English
- ISSN :
- 1365-2672
- Volume :
- 133
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 35946597
- Full Text :
- https://doi.org/10.1111/jam.15746