Back to Search
Start Over
Synthesis of subunits of ligandin by isolated hepatocytes.
- Source :
-
Biochimica et biophysica acta [Biochim Biophys Acta] 1987 Jun 15; Vol. 929 (1), pp. 1-4. - Publication Year :
- 1987
-
Abstract
- The pulse-chase technique was employed to determine the synthesis of the subunits of ligandin (glutathione S-transferase 1-2) by isolated hepatocytes. Ligandin comprised 2.5-3% of the total proteins synthesized. A slightly higher incorporation of [35S]methionine into the 22 k than the 25 k subunit was observed. However, the ratio of [35S]methionine incorporation into the subunits remained constant throughout the chase period, suggesting that, in spite of the considerable sequence homology, the conversion of 25 k to 22 k subunit does not occur in vivo.
Details
- Language :
- English
- ISSN :
- 0006-3002
- Volume :
- 929
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochimica et biophysica acta
- Publication Type :
- Academic Journal
- Accession number :
- 3593770
- Full Text :
- https://doi.org/10.1016/0167-4889(87)90233-3