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Architecture and self-assembly of the jumbo bacteriophage nuclear shell.
- Source :
-
Nature [Nature] 2022 Aug; Vol. 608 (7922), pp. 429-435. Date of Electronic Publication: 2022 Aug 03. - Publication Year :
- 2022
-
Abstract
- Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems <superscript>1</superscript> . In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors <superscript>2-4</superscript> . However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.<br /> (© 2022. The Author(s).)
- Subjects :
- Cryoelectron Microscopy
Bacteria cytology
Bacteria immunology
Bacteria metabolism
Bacteria virology
Bacteriophages chemistry
Bacteriophages immunology
Bacteriophages physiology
Bacteriophages ultrastructure
Cell Compartmentation
Viral Proteins chemistry
Viral Proteins metabolism
Viral Proteins ultrastructure
Virus Assembly
Subjects
Details
- Language :
- English
- ISSN :
- 1476-4687
- Volume :
- 608
- Issue :
- 7922
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 35922510
- Full Text :
- https://doi.org/10.1038/s41586-022-05013-4