Back to Search
Start Over
miR-20a-5p regulated SMAD6 to inhibit chondrogenesis of hDPSCs.
- Source :
-
Oral diseases [Oral Dis] 2023 Nov; Vol. 29 (8), pp. 3433-3446. Date of Electronic Publication: 2022 Aug 16. - Publication Year :
- 2023
-
Abstract
- Objectives: Chondrogenic differentiation of human dental pulp stem cells (hDPSCs) is highly promising for cartilage repair. The specific mechanism, however, still needs to be explicated.<br />Materials and Methods: In this study, we isolated hDPSCs and transfected cells with lentiviruses containing an over-expression, knock-down, or negative control of miR-20a-5p. Three-D pellet cultures of hDPSCs were used for the chondrogenic induction. Following the pellet culture period, chondrogenesis was assessed by histological and immunohistochemical analysis and expression of chondrogenic-related genes. Dual-luciferase report assay was performed to determine potential targeted genes of miR-20a-5p, and the phosphorylation levels of P65 and IκBα were explored. Animal experiments were performed to determine the effect of miR-20a-5p on cartilage regeneration.<br />Results: miR-20a-5p was showed to repress the expression of SMAD6 to inhibit the chondrogenic differentiation of hDPSCs. Accordingly, the knock-down of miR-20a-5p promoted cartilage regeneration in the osteochondral defects of rats. Mechanically, it is indicated that NF-κB signaling is the potential down-stream network of miR-20a-5p/Smad6 crosstalk during chondrogenic differentiation.<br />Conclusions: miR-20a-5p could target SMAD6 to activate NF-κB signaling pathway, and thus inhibit chondrogenesis of hDPSCs, which provided promising therapeutic target for cartilage defects clinically.<br /> (© 2022 Wiley Periodicals LLC.)
Details
- Language :
- English
- ISSN :
- 1601-0825
- Volume :
- 29
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Oral diseases
- Publication Type :
- Academic Journal
- Accession number :
- 35917232
- Full Text :
- https://doi.org/10.1111/odi.14331