Time-resolved transcriptome analysis of Scenedesmus obliquus HTB1 under 10% CO 2 condition.

Certain microalgal species can grow under high CO ₂ concentrations providing potential for mitigating CO ₂ pollution in flue gas produced by power plants. Microalga Scenedesmus obliquus strain HTB1 was isolated from the Chesapeake Bay and grow rapidly in a high level of CO ₂ . However, little is known about the molecular responses of HTB1 to high CO ₂ levels. Here, we investigated how HTB1 responds to 10% CO ₂ using the time-resolved transcriptome analysis. Gene expression profiles indicated that HTB1 responds quickly (in 2 h) and becomes adaptive within 12 h when exposed to 10% CO ₂ . Interestingly, certain genes of light-harvesting, chlorophyll synthesis and carbon fixation (i.e. rbcS) were up-regulated at 10% CO ₂ , and these functional responses are consistent with the increased photosynthesis efficiency and algal biomass under 10% CO ₂ . Nitrate assimilation was strongly enhanced, with amino acid biosynthesis and aminoacyl tRNA biosynthesis genes being markedly up-regulated, indicating that HTB1 actively takes up nitrogen and accelerates protein synthesis at 10% CO ₂ . Carbon metabolism including fatty acid biosynthesis and TCA cycle was enhanced at 10% CO ₂ , supporting the earlier observation of increased lipid content of Scenedesmus sp. under high CO ₂ levels. Interestingly, key genes like RuBisCO (rbcL) and carbonic anhydrase in carboxysomes did not respond actively to 10% CO ₂ , implying that exposure to 10% CO ₂ has little impact on the carbon concentrating mechanisms and CO ₂ fixation of the Calvin cycle. It appears that HTB1 can grow rapidly at 10% CO ₂ without significant metabolic changes in carbon fixation and ATP synthesis.
(© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)