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Endothelial Barrier Disruption by Lipid Emulsions Containing a High Amount of N3 Fatty Acids (Omegaven) but Not N6 Fatty Acids (Intralipid).

Authors :
Gueguen E
Morsy Y
Scharl M
Krämer SD
Zaugg M
Hersberger M
Rogler G
Wawrzyniak M
Source :
Cells [Cells] 2022 Jul 14; Vol. 11 (14). Date of Electronic Publication: 2022 Jul 14.
Publication Year :
2022

Abstract

Lipid emulsions are crucial for life-saving total parenteral nutrition (TPN). Their composition provides a high amount of essential fatty acids and calories for millions of patients with serious diseases. Nevertheless, several TPN-mediated side-effects have been reported in over 90% of patients. This project aimed to investigate the effect of a high amount of ω3 fatty acids (Omegaven <superscript>®</superscript> ) emulsion vs. a high amount of ω6 fatty acids (Intralipid <superscript>®</superscript> ) emulsions on the endothelial barrier function. EA.hy926 cell line was cultured and incubated with 0.01, 0.1, and 1 mM lipid emulsions. The influence of these lipid emulsions on the barrier function was assessed using ECIS technology, immunofluorescent microscopy, viability measurements by flow cytometry, multiplex cytokines analysis, and qRT-PCR. BODIPY staining confirmed the uptake of fatty acids by endothelial cells. ECIS measurements demonstrated that a high concentration of Omegaven <superscript>®</superscript> prevents barrier formation and impairs the barrier function by inducing cell detachment. Moreover, the expression of VE-cadherin and F-actin formation showed a reorganization of the cell structure within 2 h of 1 mM Omegaven <superscript>®</superscript> addition. Interestingly, the study's findings contradict previous studies and revealed that Omegaven <superscript>®</superscript> at high concentration, but not Intralipid, induces cell detachments, impairing endothelial cells' barrier function. In summary, our studies shed new light on the effect of lipid emulsions on the endothelium.

Details

Language :
English
ISSN :
2073-4409
Volume :
11
Issue :
14
Database :
MEDLINE
Journal :
Cells
Publication Type :
Academic Journal
Accession number :
35883643
Full Text :
https://doi.org/10.3390/cells11142202