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Selectivity and efficiency in the ligation of the pyrene:abasic base pair by T4 and PBCV-1 DNA ligases.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2022 Aug 11; Vol. 58 (65), pp. 9072-9075. Date of Electronic Publication: 2022 Aug 11. - Publication Year :
- 2022
-
Abstract
- Remarkable selectivity was observed in the ligation of 5'-phosphate 1-pyrene nucleotide terminated strands across from an abasic lesion in a DNA-templated ligation reaction by two different ligases suggesting that pyrene-terminated strands could be used in abasic site detection. Increasing ATP concentration was critical to enhancing the selectivity for this base pair with T4 DNA ligase.
- Subjects :
- Base Pairing
Molecular Structure
Pyrenes
DNA Ligases metabolism
Nucleotides
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 58
- Issue :
- 65
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 35876431
- Full Text :
- https://doi.org/10.1039/d2cc03079a