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Mechanical force-promoted osteoclastic differentiation via periodontal ligament stem cell exosomal protein ANXA3.
- Source :
-
Stem cell reports [Stem Cell Reports] 2022 Aug 09; Vol. 17 (8), pp. 1842-1858. Date of Electronic Publication: 2022 Jul 21. - Publication Year :
- 2022
-
Abstract
- Exosomes play a critical role in intracellular communication. The biogenesis and function of exosomes are regulated by multiple biochemical factors. In the present study, we find that mechanical force promotes the biogenesis of exosomes derived from periodontal ligament stem cells (PDLSCs) and alters the exosomal proteome profile to induce osteoclastic differentiation. Mechanistically, mechanical force increases the level of exosomal proteins, especially annexin A3 (ANXA3), which facilitates exosome internalization to activate extracellular signal-regulated kinase (ERK), thus inducing osteoclast differentiation. Moreover, the infusion of exosomes derived from PDLSCs into mice promotes mechanical force-induced tooth movement and increases osteoclasts in the periodontal ligament. Collectively, this study demonstrates that mechanical force treatment promotes the biogenesis of exosomes from PDLSCs and increases exosomal protein ANXA3 to facilitate exosome internalization, which activates ERK phosphorylation, thus inducing osteoclast differentiation. Our findings shed light on new mechanisms for how mechanical force regulates the biology of exosomes and bone metabolism.<br />Competing Interests: Conflict of interests The authors declare no competing interests.<br /> (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 2213-6711
- Volume :
- 17
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Stem cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 35868309
- Full Text :
- https://doi.org/10.1016/j.stemcr.2022.06.006