Overexpression of mammalian phenylalanyl-tRNA synthetase upon phenylalanine restriction.

Authors :: Lazard M
Mirande M
Waller JP
Source :: FEBS letters [FEBS Lett] 1987 May 25; Vol. 216 (1), pp. 27-30.
Publication Year :: 1987
Abstract: The effect of phenylalanine restriction on the level of expression of phenylalanyl-tRNA synthetase from cultured Chinese hamster ovary cells was investigated. By lowering the phenylalanine concentration from 200 to 2 microM, cell growth was arrested, tRNAPhe aminoacylation level was rapidly and specifically decreased and phenylalanyl-tRNA synthetase was derepressed. The progressive 2-fold elevation of phenylalanyl-tRNA synthetase level was determined by activity measurement and immunotitration. None of the other aminoacyl-tRNA synthetases tested were significantly affected.

Subjects :: Animals
Cell Line
Cricetinae
Enzyme Induction drug effects
Female
Fibroblasts metabolism
Ovary
Phenylalanine metabolism
Phenylalanine-tRNA Ligase genetics
RNA, Transfer metabolism
Amino Acyl-tRNA Synthetases biosynthesis
Phenylalanine pharmacology
Phenylalanine-tRNA Ligase biosynthesis

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