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Humibacter ginsenosidimutans sp. nov., with ginsenoside-converting activity isolated from activated sludge.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2022 Jul; Vol. 72 (7). - Publication Year :
- 2022
-
Abstract
- A novel Gram-stain-positive, thin rod-shaped, non-motile, aerobic and creamy-white pigmented bacterium (designated strain WJ7-1 <superscript>T</superscript> ) was isolated from activated sludge sampled in Suwon, Republic of Korea. 16S rRNA gene sequence analysis indicated that the isolate belongs to the genus Humibacter , family Microbacteriaceae , with a sequence similarity of 97.9 % to its nearest neighbour Humibacter albus KACC 20986 <superscript>T</superscript> . Strain WJ7-1 <superscript>T</superscript> contained menaquinone (MK)-10 (16.0 %), 11 (48.5 %) and 12 (35.5 %) as major respiratory quinones. The predominant cellular fatty acids (>15 %) were anteiso-C <subscript>17 : 0</subscript> , iso-C <subscript>16 : 0</subscript> and anteiso-C <subscript>15 : 0</subscript> . The peptidoglycan of strain WJ7-1 <superscript>T</superscript> contained the diagnostic diamino acid ornithine and 2,4-diaminobutyric acid alanine. Alanine, glutamic acid and glycine were also present in the cell wall. The characteristic whole-cell sugars present were glucose, galactose, xylose and rhamnose. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and two unidentified phospholipids. Strain WJ7-1 <superscript>T</superscript> possessed ginsenoside-converting ( β -glucosidase) activity, which enabled it to transform ginsenoside protopanaxadiol-type Rb <subscript>1</subscript> (one of the dominant active components of ginseng) to compound K. The genome size of strain WJ7-1 <superscript>T</superscript> has 4.2 Mbp and the G+C content is 68.3 mol%. Average nucleotide identity, amino acid identity and digital DNA-DNA hybridization values between strain WJ7-1 <superscript>T</superscript> and the closely related strain were 79.8, 36.1 and 23.5 %, respectively, indicating that strain WJ7-1 <superscript>T</superscript> represents a novel species of the genus Humibacter . Strain WJ7-1 <superscript>T</superscript> could be distinguished from the other members of the genus Humibacter by a number of chemotaxonomic and phenotypic characteristics. Based on polyphasic taxonomic analysis, strain WJ7-1 <superscript>T</superscript> (=KACC 19729 <superscript>T</superscript> =LMG 30802 <superscript>T</superscript> ) represents a novel species within the genus Humibacter , for which the name Humibacter ginsenosidimutans sp. nov. is proposed.
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 72
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 35816438
- Full Text :
- https://doi.org/10.1099/ijsem.0.005442