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Epidemiology of bla CTX-M -Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010-2017.
- Source :
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Frontiers in microbiology [Front Microbiol] 2022 Jun 17; Vol. 13, pp. 865254. Date of Electronic Publication: 2022 Jun 17 (Print Publication: 2022). - Publication Year :
- 2022
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Abstract
- Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of bla <subscript>CTX-M</subscript> genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, from 2010 to 2017. A total of 221 bla <subscript>CTX-M</subscript> -positive isolates were recovered from 1,263 S. Typhimurium isolates from the facal samples of diarrhoea patients in 45 general hospitals from 11 cities. The most popular CTX-M gene was bla <subscript>CTX-M-55</subscript> (39.6%, 72/182) in the CTX-M-1 group, followed by bla <subscript>CTX-M-14</subscript> (22.5%, 41/182) and bla <subscript>CTX-M-65</subscript> (19.2%, 35/182) in the CTX-M-9 group. The isolates that carried bla <subscript>CTX-M-9G</subscript> had significantly higher resistance rates to multiple antibacterials compared with bla <subscript>CTX-M-1G</subscript> ( p â<â0.01). Meanwhile, PFGE analysis not only showed the clonal transmission of bla <subscript>CTX-M-55/14/65</subscript> -positve isolates of diarrhoeal outpatients' origins from different hospitals in Guangdong province, but also the characteristic of bla <subscript>CTX-M-55/14/65</subscript> -positve isolates' bacterial persistence. Multilocus sequence typing (MLST) analysis indicated that these S. Typhimurium isolates possessed ST34 and ST19. Furthermore, genomic Beast phylogenomic analysis provided the evidence of a close relationship of bla <subscript>CTX-M</subscript> -positive S. Typhimurium isolates between the outpatients and pork. Most bla <subscript>CTX-M-55/14/65</subscript> genes were transmitted by non-typeable or IncI1/IncFII/IncHI2 plasmids with the size of ranging from ~80 to ~280âkb. Moreover, whole-genome sequencing (WGS) analysis further revealed that bla <subscript>CTX-M-55/14/65</subscript> coexisted with other 25 types of ARGs, of which 11 ARGs were highly prevalent with the detection rates >50%, and it first reported the emergence of bla <subscript>TEM-141</subscript> in S. Typhimurium. This study underscores the importance of surveillance for bla <subscript>CTX-M</subscript> -positive microbes in diarrhea patients.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2022 Jiang, Ke, Wu, Wang, Fang, Sun, Wang, Lei, Shao and Liao.)
Details
- Language :
- English
- ISSN :
- 1664-302X
- Volume :
- 13
- Database :
- MEDLINE
- Journal :
- Frontiers in microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 35783425
- Full Text :
- https://doi.org/10.3389/fmicb.2022.865254