Bessel-beam illumination Raman microscopy.

Authors :: Bando K
Yabuuchi S
Li M
Kubo T
Oketani R
Smith NI
Fujita K
Source :: Biomedical optics express [Biomed Opt Express] 2022 May 03; Vol. 13 (6), pp. 3161-3170. Date of Electronic Publication: 2022 May 03 (Print Publication: 2022).
Publication Year :: 2022
Abstract: We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.<br />Competing Interests: KB and KF are the inventors of a patent on Bessel-illumination Raman microscopy submitted by Osaka University.<br /> (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)