Structure and gene cluster of the O-antigen of Enterobacter cloacae G2559.

Authors :: Wang M
Filatov AV
Shashkov AS
Liu J
Perepelov AV
Source :: Carbohydrate research [Carbohydr Res] 2022 Sep; Vol. 519, pp. 108612. Date of Electronic Publication: 2022 Jun 09.
Publication Year :: 2022
Abstract: The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D <superscript>1</superscript> H and <superscript>13</superscript> C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established. The O-antigen gene cluster of Enterobacter cloacae G2559 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure.<br /> (Copyright © 2022 Elsevier Ltd. All rights reserved.)