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Pleiotropic effects of trans-regulatory mutations on fitness and gene expression.
- Source :
-
Science (New York, N.Y.) [Science] 2022 Jul; Vol. 377 (6601), pp. 105-109. Date of Electronic Publication: 2022 Jun 30. - Publication Year :
- 2022
-
Abstract
- Variation in gene expression arises from cis- and trans-regulatory mutations, which contribute differentially to expression divergence. We compare the impacts on gene expression and fitness resulting from cis- and trans-regulatory mutations in Saccharomyces cerevisiae , with a focus on the TDH3 gene. We use the effects of cis-regulatory mutations to infer effects of trans-regulatory mutations attributable to impacts beyond the focal gene, revealing a distribution of pleiotropic effects. Cis- and trans-regulatory mutations had different effects on gene expression with pleiotropic effects of trans-regulatory mutants affecting expression of genes both in parallel to and downstream of the focal gene. The more widespread and deleterious effects of trans-regulatory mutations we observed are consistent with their decreasing relative contribution to expression differences over evolutionary time.
Details
- Language :
- English
- ISSN :
- 1095-9203
- Volume :
- 377
- Issue :
- 6601
- Database :
- MEDLINE
- Journal :
- Science (New York, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 35771906
- Full Text :
- https://doi.org/10.1126/science.abj7185