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Genetic characterization of a novel Ehrlichia chaffeensis genotype from an Amblyomma tenellum tick from South Texas, USA.

Authors :
Arroyave E
Quade B
Mendell NL
Blanton LS
Bouyer DH
Source :
Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2022 Sep; Vol. 13 (5), pp. 101990. Date of Electronic Publication: 2022 Jun 14.
Publication Year :
2022

Abstract

Ehrlichia chaffeensis is the causative agent of human monocytotropic ehrlichiosis (HME), a disease that ranges in severity from mild to fatal infection. Ehrlichia chaffeensis is maintained in a zoonotic cycle involving white-tailed deer (Odocoileus virginianus) as the main vertebrate reservoir and lone star ticks (Amblyomma americanum) as its principal vector. Through complete genomic analysis from human ehrlichial isolates and DNA sequences obtained from deer and tick specimens, nine strains of E. chaffeensis have been characterized. Few studies have examined the genetic diversity of E. chaffeensis in ticks, and some of these investigations have identified that the genetic sequences coincide with the circulating strains reported so far. Here, we report the first evidence of E. chaffeensis DNA from an unfed Amblyomma tenellum (formerly Amblyomma imitator) collected in South Texas. We characterized the genetic variation of this E. chaffeensis genotype using conserved gene markers such as rRNA, dsb, and groEL. We also used gene targets useful to distinguish genotypes, such as the variable length PCR target gene (VLPT) and 120-kDa gene, encoding the tandem-repeat proteins TRP32 and TRP120, respectively. Our results suggest a novel E. chaffeensis genotype that exhibited greater variability than other genotypes of E. chaffeensis and highlights the role for A. tenellum as a potential vector of E. chaffeensis.<br /> (Copyright © 2022 Elsevier GmbH. All rights reserved.)

Details

Language :
English
ISSN :
1877-9603
Volume :
13
Issue :
5
Database :
MEDLINE
Journal :
Ticks and tick-borne diseases
Publication Type :
Academic Journal
Accession number :
35763959
Full Text :
https://doi.org/10.1016/j.ttbdis.2022.101990