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Alterations in CD39/CD73 axis of T cells associated with COVID-19 severity.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2022 Aug; Vol. 237 (8), pp. 3394-3407. Date of Electronic Publication: 2022 Jun 27. - Publication Year :
- 2022
-
Abstract
- Purinergic signaling modulates immune function and is involved in the immunopathogenesis of several viral infections. This study aimed to investigate alterations in purinergic pathways in coronavirus disease 2019 (COVID-19) patients. Mild and severe COVID-19 patients had lower extracellular adenosine triphosphate and adenosine levels, and higher cytokines than healthy controls. Mild COVID-19 patients presented lower frequencies of CD4 <superscript>+</superscript> CD25 <superscript>+</superscript> CD39 <superscript>+</superscript> (activated/memory regulatory T cell [mTreg]) and increased frequencies of high-differentiated (CD27 <superscript>-</superscript> CD28 <superscript>-</superscript> ) CD8 <superscript>+</superscript> T cells compared with healthy controls. Severe COVID-19 patients also showed higher frequencies of CD4 <superscript>+</superscript> CD39 <superscript>+</superscript> , CD4 <superscript>+</superscript> CD25 <superscript>-</superscript> CD39 <superscript>+</superscript> (memory T effector cell), and high-differentiated CD8 <superscript>+</superscript> T cells (CD27 <superscript>-</superscript> CD28 <superscript>-</superscript> ), and diminished frequencies of CD4 <superscript>+</superscript> CD73 <superscript>+</superscript> , CD4 <superscript>+</superscript> CD25 <superscript>+</superscript> CD39 <superscript>+</superscript> mTreg cell, CD8 <superscript>+</superscript> CD73 <superscript>+</superscript> , and low-differentiated CD8 <superscript>+</superscript> T cells (CD27 <superscript>+</superscript> CD28 <superscript>+</superscript> ) in the blood in relation to mild COVID-19 patients and controls. Moreover, severe COVID-19 patients presented higher expression of PD-1 on low-differentiated CD8 <superscript>+</superscript> T cells. Both severe and mild COVID-19 patients presented higher frequencies of CD4 <superscript>+</superscript> Annexin-V <superscript>+</superscript> and CD8 <superscript>+</superscript> Annexin-V <superscript>+</superscript> T cells, indicating increased T-cell apoptosis. Plasma samples collected from severe COVID-19 patients were able to decrease the expression of CD73 on CD4 <superscript>+</superscript> and CD8 <superscript>+</superscript> T cells of a healthy donor. Interestingly, the in vitro incubation of peripheral blood mononuclear cell from severe COVID-19 patients with adenosine reduced the nuclear factor-κB activation in T cells and monocytes. Together, these data add new knowledge to the COVID-19 immunopathology through purinergic regulation.<br /> (© 2022 Wiley Periodicals LLC.)
- Subjects :
- Adenosine blood
Adenosine Triphosphate blood
Annexins
CD28 Antigens metabolism
Cytokines blood
GPI-Linked Proteins metabolism
Humans
Leukocytes, Mononuclear metabolism
Receptors, Purinergic
Signal Transduction
5'-Nucleotidase metabolism
Apyrase metabolism
COVID-19 immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 237
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 35754396
- Full Text :
- https://doi.org/10.1002/jcp.30805