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Proposal of an in vitro thrombus-growth model for evaluating anticoagulants.
- Source :
-
Drug discoveries & therapeutics [Drug Discov Ther] 2022 Jul 20; Vol. 16 (3), pp. 135-138. Date of Electronic Publication: 2022 Jun 26. - Publication Year :
- 2022
-
Abstract
- The general anticoagulant evaluation requires high expense equipment, reagents, and space. Therefore, not all laboratories can perform research related to anticoagulant. In this study, we propose a novel simple method "in vitro thrombus-growth model" that can evaluate anticoagulant ability by measuring weight. The in vitro thrombus-growth model is prepared by creating a "growth-clot" with citrate plasma, calcium chloride, and thrombin, and then pouring new citrate plasma onto it. The prepared growth-clots were increased in volume in citrated human plasma, including surpluses calcium chloride, which was released slowly, leading to clot coagulation around the plasma. As a result of evaluating the anticoagulant ability of direct thrombin inhibitor using this in vitro thrombus-growth model, it was confirmed that clot growth was suppressed in a concentration-dependent manner. Therefore, this thrombus-growth model is useful as a primary anticoagulant test that can to discover compounds with anticoagulant activity perform in any laboratory.
Details
- Language :
- English
- ISSN :
- 1881-784X
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Drug discoveries & therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 35753800
- Full Text :
- https://doi.org/10.5582/ddt.2022.01040